Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 19, Issue 3, 2017; Page No.(496-508)

IDENTIFICATION AND CHARACTERIZATION OF THERMOPHILIC AND PECTINOLYTIC BACTERIA ISOLATED FROM GUNUNG PANCAR HOT SPRING BOGOR, INDONESIA

GALIH AYHUSTA LARAS, MIMIN NUR HANDAYANI, LAURA NAVIKA YAMANI,PURKAN AND NI NYOMAN TRI PUSPANINGSIH

Abstract

Thermophilic bacteria produce thermo stable enzymes that are essential in industrial processes and biotechnology, one of these is a pectinolytic enzyme. Most of the pectinolytic bacteria have growth at temperatures below 40 °C, and optimal up to a temperature of 60 °C. Pectinolytic enzyme serves to break down the complex polysaccharide from plant tissue into simple molecules such as galakturonic acids used widely in the food industry, for extraction of fruit by reducing turbidity and bitterness of fruit juices, oil extraction, fermentation of coffee and tea, textile industry, and processing of fiber plants and paper production. This study aims to identify phylogenetic of thermophilic bacteria that carry pectinolytic enzymes from the hot springs of Mount Pancar, Bogor with the identification and characterization of 16S rRNA pectinolytic enzymes as well as activity against specific substrate pectin. 16S rRNA gene amplification process was performed by PCR and amplicon obtained was determined nucleotide sequences by sequencing. The results of phylogenetic analysis showed that the isolates obtained have high homology with Anoxybacillus flavithermus, while the other isolates have homology with Geobacillus kastopilus. Pectinase enzyme activity was measured using the DNS method and characterization of enzyme activity was carried out at a temperature of 40-90°C and pH 3-10, showing optimum activity at 60°C and 70°C at pH 5.0 and 6.0.

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