Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 19, Issue 3, 2017; Page No.(560-564)

GENETIC VARIATION OF GYRINOPS VERSTEGII ORIGINATED FROM PAPUA BASED ON RAPD

RIMA HERLINA S. SIBURIANA, ULFAH J. SIREGARB AND ISKANDAR Z. SIREGARB

Abstract

Gyrinops verstegii is one of the potential tree species for production of agar wood in the eastern part of Indonesia. In its natural habitat, this species is found to be morphologically variable. This morpho-variation may reflect also the degree of genetic diversity as usually observed in many plant species. However, genetic information of Gyrinops verstegii is still lacking. A genetic inventory was therefore conducted, aiming at estimating the genetic variation using population samples from low and high altitudes, i.e. Kebar and Manokwari populations. Genetic analyses were performed using DNA marker RAPD following standard procedures. Results showed that the genetic variability of Kebar population (He= 0, 2944) was higher than that of Manokwari (He= 0.2357). AMOVA analysis showed that most of the variation is stored in individual level contributed to 89 % of the total variation. Dendrogram analysis showed that the reproductive populations, i.e mother tree and its progenies of each location, were grouped together. The molecular information can be used for scientific consideration in developing strategies for conservation and breeding.

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