Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 19, Issue 3, 2017; Page No.(654-656)

EFFECT OF CULTURE MEDIA ON SCLEROTINIA SCLEROTIORUM FOR MOST SUITABLE MYCELIA GROWTH UNDER IN-VITRO CONDITIONS

PRAMOD KUMAR FATEHPURIA, RAJNI SINGH SASODE, DEVASHISH R. CHOBE AND VEERENDRA GUPTA

Abstract

This study aim was to investigate the occurred maximum growth of Sclerotinia sclerotiorum with keeping this view in mind present experiment was conducted at Department of Plant Pathology, College of Agriculture, RVSKVV, Gwalior (M.P.) with Seven solid culture Media viz., Malt extract apple agar, Malt extract agar, Czapek's Dox agar, Potato carrot agar, Pea dextrose agar, Mustard dextrose agar, and Potato dextrose agar were evaluated against S. sclerotiorum. The radial mycelial growth recorded started from 3rd, 5th and 7th days after inoculation. The experiment was laid out in Complete Randomized Design with three replications. The data revealed that maximum radial mycelial growth 84.67mm was reported in Potato dextrose agar as semi-solid media, while, the least radial mycelial growth 38.33 mm and were occurred in Czapek’s Dox medium. The remains other medium were recorded radial mycelial growth and sclerotia in ascending order of mediums viz., Mustard dextrose agar (73.33 mm ), Potato carrot agar (72.67 mm), Malt extract apple agar (64.67 mm), Malt extract agar (61.33 mm), Pea dextrose agar (59.33 mm), respectively. No significance differences radial mycelia growth and number of sclerotia were observed between all evaluated Medias except Potato dextrose agar and Czapek’sDox medium. PDA medium was noticed for best radial mycelia growth and maximum number of sclerotia at 25± 1°C significantly occurred.

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