Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 19, Issue 3, 2017; Page No.(657-661)

ISOLATION AND MOLECULAR IDENTIFICATION OF OIL DEGRADING BACTERIA FROM WORKSHOP YARD SOIL IN KOLLAM DISTRICT, INDIA

D.S. SAYI, SARANYA SYAMALAN AND K. VINOD KUMAR

Abstract

Release of hydrocarbons generated from crude oil into the environment is one of the main causes of pollution in water and soil. This could lead to a major imbalance in biotic and abiotic units of ecosystem if not attempted to remediate it. Locally adaptive oil degrading bacteria have to be identified for improving the efficiency of microbial bioremediation technology in future. Therefore, the present study was focused on screening and isolation of geographically adaptive oil degrading bacteria from Kerala State Road Transport Corporation’s (KSRTC) mechanic workshop, situated in Kollam district, Kerala, India (8.891194°N 76.585128°E). Bacterial colonies from oil contaminated soil samples were isolated, which were able to grow in medium containing engine oil as a sole carbon source indicating oil degrading property. One of the isolate (SNCK-2) was further subjected to 16S rDNA sequencing and identified belonging to Bacillus cereus. Since Bacillus spp. is well studied for its oil degrading properties. Current strain “Bacillus cereus” can be considered as the first identified and geographically adaptive oil degrading bacteria from Kollam district and which created a preliminary data in this aspect. Further research is needed to explore its bioremediation potential.

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