CHARACTERIZATION OF AMYLASE PRODUCING BACILLUS SPECIES ISOLATED FROM RHIZOSPHERE SOIL OF POMEGRANATE PLANT AND OPTIMIZTION OF PARAMETERSRAJENDRABHAI D. VASAITAbstract Soil samples from rhizosphere of pomegranate plants were collected and analysed for bacterial content. Soft, cream white colour colony forming organism was studied and analysed for production of amylases enzyme. The organism was found potential producer of amylase. After study of morphological, biochemical and genetical characteristics organism was identified as Bacillus species. The isolated organism was phylogenetically characterized and found close relation with species Licheniformis. Isolated organism was used for production of amylases enzyme using production medium. During quantitative screening maximum enzyme activity was measured 112U/mL after 32 hours at 30 oC. Optimization of amylase production was conducted using parameters such as carbon and nitrogen source, temperature, pH, inoculum size and reaction temperature for enzyme activity. The amylase enzyme production by isolated Bacillus species was increased at optimized parameters. Increased enzyme activity of 138.8 U/mL was measured at all optimized parameters after 32 hours at 30 oC. Optimization study revealed the enhancement of production of amylase enzyme may be due to presence of favourable conditions and medium components.
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