Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 19, Nov. Suppl, Issue 2017; Page No.(56-63)

ALPHA AMYLASE PRODUCTION BY SOLID STATE FERMENTATION OF COLOCASIA ESCULENTA (L.) SCHOTT USING ASPERGILLUS LUCHUENSIS BS1

S. BALAJI, V. VEERABHUVANESHWARI, N. SARASWATHY, P. RAMALINGAM AND P. MUTHUKUMARAN

Abstract

In this present investigation Colocasia esculenta (L.) schott. corm was utilized as a carbon source to produce α-amylase by Aspergillus luchuensis bs1 under solid state fermentation. The factors influencing alpha amylase production was statistically analysed by experimenting one variable at a time. The study revealed maximum alpha amylase production (193kU) at taro corm to nutrient salt solution ratio (1:3), temperature (40°C), pH 5.6, incubation period (5 days), yeast extract (0.5%) as complex nitrogen source and nutrient salt solution as moistening agents. The alpha amylase preparation recorded finest activity at pH 5.5, 30 minutes of contact time, temperature stability at 40°C and 87% specificity to potato starch. The desized cotton fabric showed 9.5% weight loss, 5 seconds of absorbency time and 8 rating in Tegewa analysis for 1.8 % enzyme preparation. The study proposes a novel carbon source to produce alpha amylase and an enzyme preparation for desizing sized cotton fabric in minimal concentration.

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