Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 19, Issue 4, 2017; Page No.(913-924)

STUDY OF VARIABILITY IN WILD ISOLATES OF PLEUROTUS THROUGH MULTILOCUS ENZYME ELECTROPHORESIS (MLEE)

YASHVANT PATEL, SANJAY K. VISHWAKARMA, ANKIT K. PATEL AND

Abstract

Conservation of fungal diversity is critical for ecosystems to function, and if we conserve fungi, they will also continue to provide important resources in prospecting for new species, genes and enzymes for newer products and services. And for any meaningful bio-conservation exercise to succeed the primary emphasis should obviously be on the assessment of their ‘genetic variability’. In the present study variability amongst the native isolates of Pleurotus species was measured through isozyme profiling based on MLEE by sixteen enzymes. A total of 39 isolates were identified as Pleurotus species by their morphological characteristics out of 60 collected isolates. Mycelial growth rate measured and it was in range of 0.9 to 7.5 mm/day. A total of 23 consistent scorable bands, called as loci, were obtained and upon further analyses all the loci were found polymorphic; they exhibited in a total of 480 alleles with an average value of 20.86 alleles per locus. The total number of observed allele was 2.000 whereas the total number of effective alleles ranged from 1.1413 to 1.9969 with a mean ± standard deviation of 1.6810±0.2538. The magnitude of genetic differentiation among the isolates was 0.3907±0.1013, indicating that all loci differ between all pairs of enzyme loci. Adh-1 loci showed a minimum gene diversity of 0.1238 while maximum diversity of 0.4992 was shown by two loci (Est-6, G6pdh-2). Shannon`s information index was found 0.5744±0.1160. Genetic distances were calculated on the basis of similarity coefficient matrix among all isolates involved in the cluster analysis produced in the form of dendrogram. Hence, MLEE is still a very simple and reproducible technique to assess the diversity in organisms who`s genome sequence is not released.

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