Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 19, Issue 4, 2017; Page No.(925-928)

SEQUENCING AND IN SILICO ANNOTATIONS OF A NOVEL PSEUDOMONAS STRAIN

SHWETA SACHAN, PARUL JOHRI, MALA TRIVEDI AND ADITI SINGH

Abstract

Symbioses of soil micro-organisms have proved to be of fundamental importance for plant or crop adaptation. In order to discover a microbial species for lipase enzyme production, we isolated the organisms found in the soil of sugarcane field. The strains were isolated, identified and a total of six different bacterial strains were obtained. All were assessed for their lipolytic potential, in which only one gram negative strain was found to be most active. The bacteria was identified as Pseudomonas and further confirmed by 16s rRNA sequencing technique. The sequence came out to be a novel sequence and was deposited in the global nucleotide sequence repository Genbank database of NCBI (accession ID: KX946966.1). The sequence was additional annotated using bioinformatics tools. The sequence statistic was calculated, AT%-GC%, number of codons, translational frames, phylogenetic analysis, homology search and restriction site identification was done for the curation of meta data of the sequence. The number of codons in the sequence was 479. The Blast result showed that our sequence was homologous with the Pseudomonas aeruginosa species, giving 0 e value and 2630 as bit score. This sequence can further be analyzed for its biochemical and physiological characteristics and could be commercially used in various industries for enzymatic production.

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