Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

IN VITRO MASS MULTIPLICATION IN ANTHURIUM ANDREANUM (HORT) CULTIVARS THROUGH CALLUS MEDIATED ORGANOGENESIS

PRAKASHA, D. P., RAMYA, G. AND SRINIVASALU, G.B.

Abstract

This study presents mass multiplication system through callus mediated organogenesis in popular Anthurium andreanum cv. Sun Glow, Chaco and Pistachio. Explants were sterilized, innoculated in callus initiation medium (MS salts, inositol 100 mg/L, thymine HCL 0.4mg/L, sucrose 30gm/L, 2,4-D 0.2mg/L, BAP 1 mg/L and agar 0.60 g/L) and incubated in dark. Creamy and compact callus initiated in cut ends along the veins of explants in 30-35 days of inoculation, it was sub cultured at 30 days interval for 6-7 cycles under dark incubation for maximization. Petiole with leaf showed highest callusing response (90±6.87) in cv. Sun Glow and Leaf lamina with mid rib explants showed highest callusing response (65.71±6.05) in cv. Pistachio. Highest callus multiplication ratio (4.35) was observed in cv. Pistachio and highest shoot buds per clump (26.29) was observed in cv. Sun Glow. After seventh subculture, the callus was incubated in dark for 45 days to shoot bud elongation. Highest no. of shoot buds elongation was observed in cv. Sun Glow 21.11-± 2.31 and highest height of shoot was observed in cv. Chaco (6.98 ± 0.51). Then, shoots were separated, inoculated in shoot multiplication/elongation medium (MS salts, inositol 100 mg/L, thymine HCL 0.4mg/L, pyridoxine HCL 1 mg/L, calcium pentothenate 1 mg/L, Nicotinic acid 1mg/L, BAP 1.5mg/L, IAA 0.3mg/L) and incubated in light. Remaining callus was sub cultured in callus multiplication medium and incubated in dark. This continued for 5 subculture callus and shoot multiplication ratio was 1.81-2.31 and 1.45-2.46 in each cycle with both highest callus multiplication ratio and shoot multiplication ratio in Sun Glow. 12-21 shoots elongated from a bottle with highest shoot height was observed in cv. Chaco and Shoots attained 5.31-6.98 cm and highest shoot height was observed in cv. Chaco. 100 shoots were rooted on rooting medium (Shoot elongation medium). Rooted Shoots were washed and hardened in 1:1 ratio soil rite and cocopeat mix in portrays in green house, and then transferred to beds prepared with coconut coirpith in poly house for next level of hardening. At first level of hardening in green house 73.51-85.42% of plants survived in various cultivars and at second level of hardening 90.38-93.603% plants survived. Later plants were transferred to poly covers or pot with potting mixture, they appeared normal and flowered.