BIODEGRADATION OF CHLORPYRIFOS BY NOVEL PROTOPLAST FUSANTSSARAH AGGAG, RAMY SHOMAN, MOUNIR MOUSSA AND MOHAMED YACOUTAbstract Chlorpyrifos is one of the most widely used organophosphate pesticides in agricultureall over the world. Chlorpyrifos considered dangerous to humans by the World Health Organization. The objective of this work was to degrade this pesticide using three fusants from common bacteria that found in farmland (Bacillus megaterium, Rhizobium legumino-sarum and Pseudomonas fluorescens). Those strains had been chosen for their efficiency in bio-control. The protoplast fusion, which appealed to be a novel way in genetic engineering, were examined for their abilities in biodegradation. Molecular studies were applied also to identify the genetic correlation between fusants and their parents, such as RAPD and SDS-PAGE. Results in the present study showed stability in those fused strains and an increase of Chlorpyrifos degradation. These novel fusants strains could be used in a large scale as they proved to be environmentally safe and effective in biodegradation of pesticides.
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