Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

CHARACTER MOTILITY OF LIQUID SEMEN ON ONGOLE CROSSBREED (PO), BALI AND MADURA BULLS WITH DIFFERENT DILUENTS AT COLD STORAGE

DIAN RATNAWATI, NURUL ISNAINI AND TRINIL SUSILAWATI

Abstract

Motility of spermatozoa is one of the parameters used to determine the fertility status of the bulls. The purpose of this study is to measure sperm motility in liquid semen using epididymis Caudal Plasma-2 (CEP-2) diluent on Ongole crossbreed (PO), Bali and Madura bulls. This study used 6 bulls consisted of 2 PO bulls, 2 Bali bulls and 2 Madura bulls which is located in Beef Cattle Research Institute, Grati Pasuruan. Semen collection was done by using an artificial vagina and then proceed with the analysis of fresh semen and liquid semen prosessed. Observation of liquid semen quality conducted until the 5th day. Examination of liquid semen motility used Computerized Assisted Semen Analysis (CASA). Parameters measured include: fresh semen quality (volume, pH, colour, consistency, concentration, mass movement, viability, motility, totally motile sperms (TSM), and abnormalities of spermatozoa) and liquid semen quality (motility, progressive motility, velocity curve is linear ( VCL), velocity straight linear (VSL), velocity average pathway (VAP), linearity (LIN), straighness (STR), wobble (WOB), hyperactivity (H), amplitude of lateral head (ALH) and beat cross frequency (BCF). Analysis data used Minitab 17 and SPSS 16. Motility parameters of liquid semen with CEP-2 diluent on PO, Bali and Madura bulls during cold storage showed almost the same value. Bali cattle and PO indicate motility and progressive motility higher than Madura bulls. Velocity (VCL, VSL and VAP) of Bali and Madura bulls higher than PO. Value of LIN and ALH were relatively stable during cold storage. Value of STR fluctuates in every breed and WOB value indicates a downward trend in all breeds during cold storage. Value of BCF on PO bulls was higher than Bali and Madura. The usage of CEP-2 diluent in the processing of liquid semen can support sperm motility on PO, Bali and Madura bulls until the 5th days of cold storage.