Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 20, Issue 1, 2018; Page No.(128-136)

CHARACTERIZATION OF ENZYME AND LIPASE GENE OF LACTOCOCCUS GARVIEAE FROM OIL CONTAMINATED SOIL

SRI SUMARSIH, MAYLINA ILHAMI KHURNIYATI, ANDRE PRATAMA AND NI NYOMAN TRI PUSPANINGSIH

Abstract

This research aims to characterize lipase of Lactococcus garvieae isolated from soil of palm oil mill effluent (POME). Screening of lipolytic bacterial was performed in Rhodamin-B agar plate and olive oil as a substrate. The lipolytic bacteria was characterized by morphology, physiology, and molecular. The lipase activity in various pH and temperature were determined toward p-Nitrophenyl palmitate substrate. The gene fragment of lipase was amplified by PCR method using specific degenerate primer for lipase group IV (HSL). The result of this research showed that one of lipolytic bacteria isolates L49 isolate had highest activity was identified as Lactococcus sp. The lipase from Lactococcus sp. Show maximum activity of 9.33 U/mL at pH 7 and temperature 50 oC. Molecular analysis 16S gene of lipolytic bacteria had 85 % homology to Lactococcus garvieae strain FMA401. The fragment lipase gene was 248 bp had 95 % homology with Acinetobacter baumanii strain ab736.

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