Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 20, Issue 1, 2018; Page No.(231-238)

ISOLATION AND CHARACTERIZATION OF SOIL BACTERIA POPULATION FROM AGRICULTURAL SOILS OF VISAKHAPATNAM DISTRICT, ANDHRA PRADESH, INDIA

SRI LAKSHMI BALAKRISHNAN, P.V.V. PRASADA RAO AND K. ARUNA REDDY

Abstract

Microorganisms are considered as indicators of soil health. Agricultural intensification has led to the usage of chemical fertilizers and pesticides that promise to give high yield in less time. Continuous usage of these pesticides releases various xenotoxic compounds into the environment that alters the physico- chemical properties of air, water and soil. They can also alter the normal microbial flora in agricultural soils. A total of 24 isolates of bacteria were isolated from four samples which were collected from agriculture practicing areas of Visakhapatnam district. From control sample a total of 63 isolates were isolated. Control soils were taken from area which is devoid of any agricultural practice. Among 24 isolates 5 isolates showed a unique morphological growth. The isolates were identified as Bacillus and Staphylococcus based on biochemical tests. Although 16s rRNA analysis were done to confirm the genus and species. Based on BLAST alignments of the sequenced nucleotide, the isolates were identified as Bacillus drentensis, B.aryabhattai, B.firmus, Staphylococcus vitulinus.

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