Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 20, Issue 1, 2018; Page No.(301-309)

MORPHOLOGICAL AND BIOCHEMICAL EVALUATION OF FREE LIVING N2 FIXING TEA RHIZOSPHERIC AND TEA SOIL BACTERIA OF NORTH BENGAL TEA GARDENS, INDIA

JAYANTA BHADURI, PRITAM KUNDU AND SUBHASH KANTI ROY

Abstract

Total 283 (126 from Terai, 12 from Teari adjoining area, 95 from Dooars and 50 from Darjeeling) isolated distinct bacterial colony from Tea soil and Tea rhizosphere of Tea gardens of Teari, Dooars and Darjeeling has been characterised morphologically for this investigation. Most of the bacterial strain in this study are Gram negative in Character. The bacterial strain DS-2-10 having characteristics similar to Azotobacter sp., DJ-1-46 to Azospirillum sp as revealed by SEM study. Among 22 isolated strain so far tested for the production of γ-glutamyl hydroxymate (μM), TS-3-15 exhibit highest response followed by DS-1-20,DJ-1-10 and DS-2-9. The highest amount of ethylene production was recorded in AS-1-4 (4.10 nmole), DS-1-20 (3.755 nmole), DS-2-10 (3.59 nmole), DJ-1-22 (1.199 nmole), DJ-1-24 (1.886 nmole). This ARA assay leads to the selection of bacterial strains to be act as potent biofertiliser. The total N content as evident from Kjeldahl Experiment, the strain DS-1-10 exhibit highest (0.09%) N content followed by DJ-1-22 (0.08%) and TS-3-15 (0.07%), DS-1-25 (0.07%), AS-1-4 (0.07%). This capacity leads to attribute a bacterial strain to act as good biofertilisers. Most of the strains produce IAA which has PGPR activity of bacterial strain to be act as Biofertiliser and exhibit tolerance to common chemical fertilizer and weedicides.

Enter your contact information below to receive full paper.
Your Name :
Email:
Phone:
City:
Cost of Full Paper: Rs.150 for Indian Nationals or $20 (USD) for international subscribers.
By clicking on Request Paper you Agree to pay the above mentioned cost per paper.