PRODUCTION AND OPTIMIZATION OF BIOSURFACTANT FROM PSEUDOMONAS AERUGINOSA SJS 5 AND SJS6VISHAL R. DHUNDALE, VIJAYSHREE M. HEMKE AND DHANJAY DESAIAbstract A total of six bacteria were isolated from the water and sediment samples collected from the hyper alkaline saline environment of Lonar crater. Out of them SJS5 and SJS6 was selected for production and, partial characterizations of biosurfactant. A Gram negative bacterium was identified as Pseudomonas aeruginosa by morphological, physiological and biochemical characterization. The bacteria grew in sodium chloride (NaCl w/v) from 0.5 to 7% and at pH 7-12. The production of a biosurfactant by Pseudomonas aeruginosa was studied to evaluate the influence of the concentration of the cotton seed oil, Soyabean oil, Coconut Oil, Sunflower Oil, Groundnut Oil, Sesame Seed Oil. The dynamics of the growth and surfactant production were showed for all the cultivation conditions studied. The organism grew and produced biosurfactant when cultured in variety of substrates at salinities up to 4 g L-1and temperatures up to 40 °C. The biosurfactant was highly stable over broad temperature from 40 to 80°C, pH 6.0-12.0, and NaCl concentration 1-4% ranges, showing excellent thermostablity and haloalkaline tolerant nature. The biosurfactants lowered the surface tension of medium and formed a stable emulsion. The biosurfactant produced by the organism emulsi?ed a range of oil with as Ground nut and coconut and soyabean oil best substrate and sun flower was the poorest. These findings further indicate that the isolate could be useful for bioremediation of hydrocarbon in industries effluent. This is valuable information for biosurfactants production and optimization has bright future towards the improvement and production of novel biosurfactants for entirely new areas of industrial and biotechnological applications.
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