Pollution Research Paper

Vol 37, May Suppl. Issue 2018; Page No.(166-170)

ISOLATION OF BIOSURFACTANT PRODUCING BACTERIA FOR OIL CLEANING FROM WATER BODIES

RAHUL, KRITIKA DHITAL, RISHA, RAJDEEP BHATTACHARJEE AND GAURAV KUMAR

Abstract

Biosurfactants are surface active biomolecules produced by a variety of microorganisms and their amphiphilic nature can reduce the tension between aqueous solutions and the hydrocarbon mixtures (such as oil). In addition, biosurfactants can emulsify and degraded a variety of hydrocarbons present in oil and thus enables their applications in the cleaning of oil from soil and water. In this context, the present study was designed for the isolation and characteristics of biosurfactant producing bacteria from the soil sample collected from Phagwara, Punjab. During this study, eight bacterial culture were isolated from four different soil samples and were screened for their property to produce biosurfactant by drop collapsing assay and oil emulsification assay. Among eight, one isolate (NK1) was found to be positive for biosurfactant production in the above methods. Further, the bacterial isolate was identifies as Bacillus sp. and exhibited promising results in the aquatic model which was designed to study the engine oil degradation by the potential biosurfactant producing bacteria.

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