Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 20, Issue 2, 2018; Page No.(523-533)

EFFECT OF DIFFERENT PHYTOHORMONES ON PLANT REGENERATION OF GLADIOLUS (GLADIOLUS HYBRIDUS HORT.) FROM CULTURED CORMEL

RAM KANYA MALVIYA, M.K. TRIPATHI, VIDHYASHANKAR M., R.P. PATEL AND ASHOK AHUJA

Abstract

Cormel explants of gladiolus (Gladiolus hybridus) was cultured on different fortifications of MS medium amended with different auxins and cytokinins in diverge concentrations as alone as well as in different combinations. Regarding in vitro response, induction medium MS2D.5B/MS2N.5B 5D (MS + 2.0 mgL-1 2,4-D + 0.5 mgL-1 BA + 30.0 gl-1 sucrose + 7.5 gl-1 agar/ MS + 2.0 mgL-1 NAA + 0.5 mgL-1 BA + 30.0 gL-1 sucrose + 7.5 gl-1 agar) promoted higher degree of callus induction. Nutrient medium MS2N.5B/MSN.5B(MS + 2.0 mgL-1 NAA+ 0.5 mgL-1 BA+ 30.0 gl-1 sucrose + 7.5 gl-1 agar/ MS + 1.0 mgL-1 NAA+ 0.5 mgL-1 BA+ 30.0 gl- 1 sucrose + 7.5 gl-1 agar) exhibited higher shoot proliferating efficiency and shoot of higher length, However, higher number of shoot (s) per explants exhibited by regeneration medium MS3B/MS2B (MS + 3.0 mgL-1 BA+ 30.0 gl-1 sucrose + 7.5 gl-1 agar/ MS + 2.0 mgL-1 BA+ 30.0 gl-1 sucrose + 7.5 gl-1 agar). In terms of in vitro rooting, higher root proliferating competence was documented on rooting medium MS.5IB.5Kn (MS + 0.5 mgL-1 IBA + 0.5 mgL-1 Kinetin + 15.0 gl-1 sucrose + 7.5 gl-1agar), while number of root (s) with higher length were recuperated on rooting medium MS.5IB (MS+0.5 mgl-1 IBA+15.0 gl-1 sucrose+ 7.5 gl-1 agar). Phenotypic ally normal plantlets were obtained and consequently transferred to pots and hardened in Environmental Growth Cabinet and Net House and transferred to field successfully.

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