Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

EFFECT OF DIFFERENT DOSES OF ANTIOXIDANT ON THE PROCESSES OF RUMEN METABOLISM IN DAIRY CATTLE WHEN VIOLATING THE FEEDING ECOLOGY

MARINA G. KOKAEVA1, VIKTORIA V.TEDTOVA, BORIS A. DZAGUROV, INNA I. KORNOUKHOVA4, ALJA N. DOEVA5, NATALYA V. LYASHENKO AND KHETAG E. KESAEV

Abstract

When cultivating forage crops to increase yield capacity great amount of nitrogenous fertilizers are applied. This factor often results in excessive accumulation of nitrates in animal feed. The research aims to study the features of cows’ rumen metabolism when reducing nitrate loads on the body through diets supplementation with preparation Mold-Zap in different doses. During the research and production experiment using four groups of lactating cows formed by the analogue scale, Mold-Zap at doses of 1, 1,5 and 2,0 kg/t feed was added to diets with the increased nitrates background. The research results were processed by Student’s method of variation statistics using the software package “Microsoft Excel”. When detoxifying the analyzed xenobiotics, the highest promoting effect on volatile fatty acids (VFA) formation had Mold-Zap feeding at a dose of 1.5 kg/t feed, so these acids concentration in molar calculus in the rumen fluid of the cows from the second test group significantly (P>0.95) exceeded that of the control by 4.5%. The highest concentration of acetic and propionic acids in the proventriculus content was in cows of the second test group, which on these indicators significantly (P>0.95) outperformed the control counterparts by 2.97 and 2.00%. Application of preparation Mold-Zap at a dose of 1.5 kg/t mixed feed for detoxification contributed in the diets of cows in the second test group relative to the control to significant (P>0.95) increase in proventriculus of proteolytic vitamin-forming Flavobacterium vitaru men colonies by 29.7%, and phagocytes count – infusoria by 24.7%. Due to high detoxification properties of Mold-Zap, the lowest nitrates and nitrites content was in the rumen liquid of animals in the second test group – 0.07 and 0.012 mmol/l, which is versus the control significantly (P>0.95) lower by 56.25 and 67.57%, respectively.