IN VITRO EVALUATION OF WATER-LESS HAND RUB SANITIZERSAVAILABLE AT LOCAL MARKET IN HOLY MAKKAH AGAINST 13ATCC BACTERIAL STRAINSSAMI S. ASHGARAbstract Cleaning hand is approved to reduce spreading of infections specially in health care premises and food industries. Waterless hand rub sanitizers have become widespread for their quickness, cost effectiveness, delicateness effects. In this laboratory-based study, waterless hand rub sanitizers available in the marketplaces at the time of commencing the study were selected based on the 99.9 bacterial reduction or antibacterial labelling. Objective was to evaluate their efficacy and synchronous killing-time against various 13 American type culture collection ATCC (strains, by the mean of determining the MIC and MBC). Results demonstrated variant antibacterial activities of 8 hand sanitizers out of 13 when tested on agar well diffusion method with zone of inhibition diameter ranges between 11-30mm. MBC assay showed bactericidal effectiveness of 4 hand sanitizers out of 13 with dissimilar time-kill between 10-50 seconds. In conclusion, water-based hand sanitizer (Dettol® foam hand wash) was experimentally the most effective product both on agar well diffusion method and time-kill test.
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