Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 20, Issue 3, 2018; Page No.(725-733)

BIOREMEDIATION OF MICROCYSTINS BY TWO NATIVE BACTERIA: BACILLUS CEREUS AND RAHNELLA AQUATILIS

F. SUMAIYA IDROOS AND PATHMALAL M MANAGE

Abstract

Microcystins (MCs) are a group of cyclic heptapeptide hepatotoxins produced by cyanobacteria. Among 70 analogues of MCs, Microcystin-LR (MC-LR) is the dominant type in Sri Lankan water bodies. Contamination of MC-LR in drinking water is a serious health issue. Thus, the present study was carried out to identify freshwater bacteria, which are capable of degrading MC-LR. BIOLOG MT2 plate assay was employed to confirm MC-LR biodegradation by the bacterium Bacillus cereus and Rahnella aquatilis which were isolated from Girandurukotte reservoir and form Ulhitiya reservoir respectively. Classic batch culture experiments showed that B. cereus and R. aquatilis removed 100% of MC-LR at 28oC within 8 and 10 days of incubation respectively. Optimized biodegradation conditions for MC-LR by B. cereus and R. aquatilis were recorded at 320C when phosphates and nitrate concentrations were 0.01ppm and 0.4 ppm respectively in experimental medium. Moreover, B. cereus and R. aquatilis were capable of degrading other MCs analogues; Microcystin-LF (MC-LF), Microcystin –LW (MC-LW), Microcystin –RR (MC-RR) and nodularin (NOD) as well. Molecular studies confirmed that B. cereus and R. aquatilis harbored MC degrading mlrA, mlrB, mlrC and mlrD genes. This is the first record for degradation of MCs and NOD by R. aquatilis.

Enter your contact information below to receive full paper.
Your Name :
Email:
Phone:
City:
Cost of Full Paper: Rs.75 for Indian Nationals or $20 (USD) for international subscribers.
By clicking on Request Paper you Agree to pay the above mentioned cost per paper.
  
Journal Issues
Vol 20, Issue 3, 2018
Vol 20, Issue 1, 2018
Vol 20, Feb. Suppl. Issue, 2018
Vol 19, Issue 4, 2017
Vol 19, Nov. Suppl, Issue 2017
Vol 19, Issue 3, 2017
Vol 19, Issue 2, 2017
Vol 19, Issue 1, 2017
Vol 18, Issue 4, 2016
Vol 18, Issue 3, 2016
Vol 18, Issue 2, 2016
Vol 18, Issue 1, 2016
Vol 17, Issue 4, 2015
Vol 17, Issue 3, 2015
Vol. 17 Special Issue 2015
Vol 17, Issue 2, 2015
Vol 17, Issue 1, 2015
Vol 16, Issue 4, 2014
Vol 16, Issue 3, 2014
Vol 16, Issue 2, 2014
Vol 16, Issue 1, 2014
Vol 15, Issue 4, 2013
Vol 15, Issue 3, 2013
Vol 15, Issue 2, 2013
Vol 15 Issue 1, 2013
Vol 14, Issue 4, 2012
Vol 14, Issue 3, 2012
Vol 20, Issue 2, 2018
Vol 14, Issue 2, 2012
Vol.14, Issue 1, 2012
Vol.13, Issue 4, 2011
Vol.13, Issue 3, 2011
Vol.13, Issue 2, 2011
Vol.13, Issue 1, 2011
Vol.12, Issue 4, 2010
Vol.12, Issue 3, 2010
Vol.12, Issue 2, 2010
Vol.12, Issue 1, 2010
Vol 11, Issue 4, 2009
Vol 11, Issue 3, 2009
Vol 11, Issue 2, 2009
Vol 11, Issue 1, 2009
Vol 10, Issue 4, 2008
Vol 10, Issue 3, 2008
Vol 10, Issue 3, 2008
Vol 10, Issue 2, 2008
Vol 10, Issue 1, 2008
Vol 9, Issue 4, 2007
Vol 9, Issue 3, 2007
Vol 9, Issue 2, 2007
Vol 9, Issue 1, 2007
Vol 8, Issue 4, 2006
Vol 8, Issue 3, 2006
Vol 08, Issue 2, 2006
Vol 08, Issue 1, 2006
Vol 7 Issue 4, 2005
Vol 07, Issue 3, 2005
Vol 07, Issue 2, 2005
Vol 6 Issue 4, 2004
Vol 6 Issue 3, 2004
Vol 6 Issue 2, 2004
Vol 6 Issue 1, 2004
Vol 5 Issue 3, 2003
Vol 5 Issue 2, 2003
Vol 04, Issue 4, 2002
Vol 04, Issue 1, 2002
Vol 3 Issue 1-2, 2001
Vol 1 Issue 1-2, 2000
Vol 1 Issue 3-4, 1999
Vol 1 Issue 1-2, 1999
Looking for Past Issues? Click here to get them!!