Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 20, Issue 3, 2018; Page No.(734-737)

MODIFICATION OF METHOD OF BACILLUS ANTHRACIS SETTING PHAGE INDICATION IN SAMPLES OF SOIL

FEOKTISTOVA N.A., VASILYEV D.A., ZOLOTUKHIN C.N., VASILYEVA Y.B., MARTYNOVA K.V., TOIGILDIN A.L., TOIGILDINA I.A., SHVIDENKO I.G. AND OBUHOV I.L.

Abstract

In this article the method research results of setting the reaction of phage titer growth aimed to determine Bacillus anthracis in non sterile samples of soil. The treatment of research soil suspension with trichloromethane at the ration of 1:10, allowed us to increase sensitivity of indication method by phage B.à. Rot YTCXA of anthrax agent till the determination of concentration equal to n x 103 CFU/g. It was established that the ideal time of exposition allowing more full cooperation of research bacteriophage B.à. Rot YTCXA with indicator crop Bacillus anthracis – IIIyr-15 is 4 hours of cultivation with pre growing of research material during 2 hours at (36±1) oC.

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