Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

EVALUATION THE BIO-STAINING AND ANTI-OXIDANT ACTIVITY OF CYMBOPOGON CITRATUS EXTRACT LEMON GRASS

TAGHREED KHUDHUR MOHAMMAD

Abstract

Plant parts have found place in biological staining of microbes due to their coloring and dying effects as natural dyes and evaluation the anti-oxidant activity. Objectives of the study was to determine and evaluation of the role of Citrates “lemon grass” extract in bio staining activity and analyze its chemical antioxidant property. Aqueous and alcoholic Cymbopogon citratus extracts wear prepared. Preliminary detection for some active ingredients in Cymbopogon citratus extracts, the lemongrass leaves were dried in the dark for one week, then 500 g of dried leaves were ground into powder then dissolved in 2000 mL of water and put them in steam distillatory, the remaining volume of deep Brown residue 200 mL after that the brown residue was transformed into large sterile petri dish and evaporated until dryness by hot air oven. The results of chemical detectors for some active substances in extracts of Cymbopogon citratus revealed that aqueous and ethanolic extracts contains many active substances the staining of all parasites by using the residue of lemon grass oil extraction when compared with control group in Concentration (10% 25%) which stained using iodine the results are approximately similar at pH 5 was better than using the same residue but in Concentration (5%) at pH5. The color of stained slides with the residue of lemon grass oil extraction was brown. In this paper the free radical scavenging activities were studied using stable free radical compound 1,1-Diphenyl-2-Picryl-hydrazil (DPPH). Results revealed that the more increasing in concentration of the extract the more scavenging percentage of the free radicals. The concentrations that were used ranged from 26.2 to 1000μg/mL, nevertheless the Ascorbic acid extract of S. officnalis significantly showed the highest antioxidant activity (86% at 1000μg/mL) among Methanol, Ethyl acetate and Hexane extracts which were 84.5.1%, 56% and 54% respectively at 1000μg/mL.