Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 20, Issue 3, 2018; Page No.(1001-1007)

BIO-ETHANOL PRODUCTION FROM BANANA PSEUDO STEM BY SIMULTANEOUS SCCHARIFICATION AND FERMENTATION PROCESS USING S.CEREVISIAE

JYOSHNAMAYEE PATRA, BISWAJIT RATH AND NABIN KUMAR DHAL

Abstract

Energy crisis and proper waste disposal is the greatest challenge of the today scenario. Biofuel from agricultural farm waste could be a propitious cybernetics though the technique has multiple challenged and restriction such as biomass supply and conduct and effectual pre-treatment methods for completely lignocellulosics delignification. Banana pseudo-stem to ethanol by cocultures of Aspergillus niger, Aspengillus fumigates, Trichoderma ressei and Saccharomyces cerevisiae by Simultaneous Saccharification and Fermentation (SSF) process. Which was studied at various temperatures (20°C to 50°C) and at different pH (4.5 to 7.5). Fermentation was done for 7 days for banana Pseudo-stem and the ethanol content was analyzed every 24 hours. For the fermentation of banana peels the optimum pH and temperature was found to be 6.5 and 30°C. With the improved temperature and pH, fermentation was conducted at different yeast concentration 3% to 12%. With changing concentration of yeast, using a 12%, 9%, 6%, 3% yeast inoculum, maximum ethanol manufactured was completely executed in 2, 3, 5 and 7 days respectively.

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