Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 20, Oct Suppl. Issue, 2018; Page No.(47-52)

ISOLATION AND CHARACTERIZATION OF PROTEASE PRODUCING BACTERIA FROM PUFFER FISH SKIN WASTE

RADEN LUKAS MARTINDRO SATRIO ARI WIBOWO, ZAENAL BACHRUDDIN, NANUNG AGUS FITRIYANTO, TOMOYUKI NAKAGAWA, TAKASHI HAYAKAWA AND AMBAR PERTIWININGRUM

Abstract

Puffer fish is one of the waste fisheries catch. It is not only can be a waste of fisheries that is difficult to degrade, but also can be used as a source of proteolytic bacteria. Thus, this innovation, i.e. enzymes produced by bacteria which cause pufferfishÂ’s skin to decay, can be used as a substitute for chemicals in tanning processes. The objective of this research was to obtain bacterial isolates from the waste of puffer fish skin (Arothon reticularis) as one of the sources of proteases. Materials used in the study were 30 pieces of 30-days fermented pufferfish skin. The results of the isolation showed that 6 of 10 kinds of strains performed caseinolytic activity by showing the clear zone in the media of Skim Milk Agar. Based on the results of SEM test, the morphological form of two types of microbes were short Bacillus, while four types of microbes were long-shaped Bacillus. The proteolytic enzymes assay showed that bacteria strain Kt had the highest specific activity (70.32 U/mg), while bacteria strain Pb had the lowest activity (32.49 U/mg). All bacteria obtained were the superior bacteria that can be used for the removal of thorns/spines from pufferfish skin in the tanning process. The proteolytic enzyme test showed that BRAW_Kt bacterial strain had the highest specific activity (70.32 U/mg) and BRAW_Pb bacterial strain had the lowest specific activity (32.49 U/mg).

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