Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 20, Oct Suppl. Issue, 2018; Page No.(109-117)

PROBIOTIC CHARACTERIZATION OF LACTIC ACID BACTERIA ISOLATED FROM RAW MILK (BUFFALLO, COW, AND GOAT) FROM WEST SUMATERA, INDONESIA

S. MELIA, FERAWATI, YUHERMAN, JASWANDI, H. PURWANTO AND E. PURWATI

Abstract

This study aims to evaluate the in vitro potential probiotic properties of lactic acid bacteria from raw milk. The lactic acid bacteria (LAB) used in this study were isolated from raw milk (buffalo, cow, and goat) sampled from different locations in West Sumatera, Indonesia. Isolates were Gram-stained and tested for catalase reaction. Probiotic properties of isolates were investigated. The selected strains were further characterized by tolerance to acidity pH 2, 0.3% of bile salt, and antibiotics susceptibility. Antimicrobial activity of the isolated strains against pathogenic bacteria was assessed using well diffusion method and hydrophobicity percentage was also examined. Finally, the selected strainsÂ’ potential as probiotic was identified by using 16S rRNA gene sequence analysis. The strains code BM 2.1 (buffalo milk), CM 1.1. (cow milk), and GM 1.1 (goat milk) were considered to be acid and bile tolerant and they exhibited antagonistic activity towards pathogenic bacteria. This research showed that the LAB from raw milks contained Probiotic bacteria, which are capable of living in digestive tract and fighting against pathogenic bacteria. The BLAST results analysis had also identified Lactobacillus fermentum strain IMAU70167 (BM 2.1), Lactobacillus fermentum strain MTCC 25067 (CM 1.1) and Lactobacillus fermentum strain NCC2970 (GM 1.1).

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