Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

QUALITY OF BOER GOAT LIQUID SEMEN ON DIFFERENT COCONUT WATER DILUENT (COCOS NUCIFERA) DURING COLD STORAGE

MUHAMMAD ADE SALIM, M.NUR.IHSAN, N. ISNAINI, A.P.A. YEKTI, T. SUSILAWATI

Abstract

Optimization of liquid semen quality of Boer goat with non-synthetic diluent raw materials using coconut water, because it has a biochemical component that functions like a synthetic diluent. The objectives of this study were to observe the quality of Boer goat liquid semen using different coconut water diluted varieties and the age of the fruit was added 10% yolk. Semen was collected from 3 Boer goat using an artificial vagina once a week. (T1) Green Young Coconut Water (viridis), (T2) Green Old Coconut Water, (T3) Red Old Coconut Water (rubescens) and (T4) Red Young Coconut Water and (P0) Tris Aminomethane as a Control. Variables analysis was individual motility, viability, abnormality and membrane integrity. Randomized completed block design (RCBD) was used with 4 treatments and 1 control and 7 replications. Data were analyzed by Analysis of Variance (ANOVA), the difference between treatments was performed with Duncan’s Multiple Range Test using Genstat 18.2 program. The results showed that treatments have a significantly effect (P <0.05) on individual motility at D0 and D2; while on the other day individual motility significant decreased (P <0.01). Further test showed that the best treatment was T0. The viability of spermatozoa showed that treatment had no effect (P> 0.05) on D0; D1 and D2 but significantly (P <0.05) in D3 and had a very significant effect (P <0.01) on D4 - D8. Further test results showed that P0 has the best viability value during cooling. The spermatozoa abnormality showed had no effect of treatments (P> 0.05) of storage D1 to D7 but significantly (P <0.05) in D0 storage. Further test results showed that T0 and T1 had the lowest values of 1.5 ± 0.6% and 1.1 ± 0.9%. The integrity membrane showed that the treatment had no effect (P> 0.05) on storage D1 to D3 but had a very significant effect (P <0.01) on storage D4 to D8. Further test results showed that T0 has the highest value on storage D4 to D8. In conclusion, the best quality is T0 up to 7 days storage with the motility = 40.0 ± 5.0%, viability = 40.7 ± 9.5%, abnormality = 1.6 + 0.6%, membrane integrity = 42.5 ± 8.55% and total motile spermatozoa = 80.0 ± 10.0 Million / mL. For the coconut water showed that (viridis) Young green coconut water stored at 2 days with the motility = 60.0 ± 7.0b%, viability = 69.3 + 8.1%, abnormality = 69.3 ± 8.1% membrane integrity = 59.8 ± 9.23% and total motile spermatozoa = 120.0 ± 15.3 million / mL.