THE CONDITIONED MEDIUM-RAT BONE MARROW DERIVED MESSENCYHMAL STEM CELL (CM-RATBMMSC) CAN INDUCE THE DIFFERENTIATION ABILITY OF NEURAL STEM AND PROGENITOR CELLS (NPCS)RATIH RINENDYAPUTRI, ARIYANI NOVIANTARI, VISTA BUDIARIATI, ULY ALFI NIKMAH AND MASAGUS ZAINURIAbstract Mesenchymal stem cell (MSC) and conditioned medium-MSC (CM-MSC) are potential therapeutic agents for the treatment of neurogenerative diseases. Conditioned medium-mesenchymal stem cell (CM-MSC) contained growth factors that can protect neuronal cells from cell death and induce differentiation of neural stem/progenitor cells (NPCs). Aims: To investigate the capability of conditioned medium rat bone marrow-derived mesenchymal stem cells (CM-rat BMMSC) induces in vitro differentiation of NPCs into astrocytes and neuron. Methods : We assessed the effects of CM-ratBMMSC to induce differentiation of NPCs by culturing cells in serum-free medium DMEM/F12 (FM), CM 100%, CM 50% and neuro basal medium with supplement (NM) for 4 days. We examined the effects on differentiation by assessing the expression of A2B5, PSANCAM, and beta-tubulin by flowcytometry, and the expressions of glial fibrilaryacidicprotein (GFAP) and neuronal nuclei (NeuN) by immunocytochemistry (ICC). Results: CM-ratBMMSC induced differentiation of NPC to astrocytes and neurons which indicated by the expression of GFAP, beta-tubulin and NeuN. Our findings showed that growth factors or neurotrophic factors such as basic fibroblast growth factor (bFGF) and nerve growth factor (NGF) in CM-ratBMMSC induces NPCs differentiations that may happen via phosphoinositide 3-kinases (PI3K)/protein or kinase B (Akt) signalling pathway. Conclusions : Growth and neurotrophic factors from CM-rat BMMSC such as bFGF and NGF increased the differentiation ability of NPCs into astrocytes (GFAP) and neurons (NeuN).
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