Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 20, Dec. Suppl. Issue, 2018; Page No.(55-61)

THE CONDITIONED MEDIUM-RAT BONE MARROW DERIVED MESSENCYHMAL STEM CELL (CM-RATBMMSC) CAN INDUCE THE DIFFERENTIATION ABILITY OF NEURAL STEM AND PROGENITOR CELLS (NPCS)

RATIH RINENDYAPUTRI, ARIYANI NOVIANTARI, VISTA BUDIARIATI, ULY ALFI NIKMAH AND MASAGUS ZAINURI

Abstract

Mesenchymal stem cell (MSC) and conditioned medium-MSC (CM-MSC) are potential therapeutic agents for the treatment of neurogenerative diseases. Conditioned medium-mesenchymal stem cell (CM-MSC) contained growth factors that can protect neuronal cells from cell death and induce differentiation of neural stem/progenitor cells (NPCs). Aims: To investigate the capability of conditioned medium rat bone marrow-derived mesenchymal stem cells (CM-rat BMMSC) induces in vitro differentiation of NPCs into astrocytes and neuron. Methods : We assessed the effects of CM-ratBMMSC to induce differentiation of NPCs by culturing cells in serum-free medium DMEM/F12 (FM), CM 100%, CM 50% and neuro basal medium with supplement (NM) for 4 days. We examined the effects on differentiation by assessing the expression of A2B5, PSANCAM, and beta-tubulin by flowcytometry, and the expressions of glial fibrilaryacidicprotein (GFAP) and neuronal nuclei (NeuN) by immunocytochemistry (ICC). Results: CM-ratBMMSC induced differentiation of NPC to astrocytes and neurons which indicated by the expression of GFAP, beta-tubulin and NeuN. Our findings showed that growth factors or neurotrophic factors such as basic fibroblast growth factor (bFGF) and nerve growth factor (NGF) in CM-ratBMMSC induces NPCs differentiations that may happen via phosphoinositide 3-kinases (PI3K)/protein or kinase B (Akt) signalling pathway. Conclusions : Growth and neurotrophic factors from CM-rat BMMSC such as bFGF and NGF increased the differentiation ability of NPCs into astrocytes (GFAP) and neurons (NeuN).

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