EARLY HEALING PHASE IN RAT’S CALVARIAL CRITICAL-SIZE DEFECT AFTER IMPLANTATION OF BOVINE CORTICAL MEMBRANEEKO WICAKSONO SUBAGIO, DAVID B. KAMADJAJA, DIANIZA AFIKANINGTYAS,ZEFRY ZAINAL ABIDIN, PRATIWI SOESILOWATI AND COEN PRAMONOAbstract Bovine pericardium collagen membrane (BPCM) had been widely used in guided bone regeneration (GBR) for alveolar bone augmentation; but it has prolonged biodegradation characteristic. A newly developed demineralized freeze-dried bovine cortical bone membrane (BCBM) is supposed to have osteogenic induction capacity. Yet, its effect on early bone healing phase needs to reveal. this study attempt to analyse the initial mechanisms of bone defect healing after implantation of BCBM in ratÂ’s calvaria criticalsize defect compared with BPCM. Thirty rats used as samples were divided into 2 groups; the experimental and control group in which BCBM and BPCM were applied over calvaria defects, respectively. Five samples were sacrificed after 3, 5, and 7 days for histology examination using Haematoxylin Eosin (HE) staining. The amount of fibroblast and angiogenesis were counted and statistically analysed using Anova and t-test with significance being p < 0.05. There were no significant differences in the amount of fibroblast in both groups of after implantation on day 3, 5, and 7. There were no significant differences in the amount of angiogenesis between the two groups at 3 and 5 day after implantation (p>0.05), but it was statistically higher than control group (p<0.05) on day 7 post implantation. Bovine cortical bone membrane shows comparable effect on early healing phase of calvaria critical-size defect compared to bovine pericardial membrane. It is, therefore, has potential for use as guided bone regeneration membrane.
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