Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 20, Dec. Suppl. Issue, 2018; Page No.(117-120)

THE EFFECT OF ALPHA TOCOPHEROL SUPPLEMENTATION ON THE EXPRESSION OF VASCULAR ENDOTHELIAL GROWTH FACTOR ON HUMANBONE MARROW MESENCHYMAL STEM CELL (IN VITRO STUDY)

IVAN TANTRA, NI PUTU MIRA, LISKA BARUS, REZA PAHLEVI, PURWATI AND COEN PRAMONO

Abstract

Surgical reconstruction is a major challenge in oral and maxillofacial surgery, autogenous bone graft considered to be the gold standard reconstruction for Bone Augmentation. Supplementation of Alpha tocopherol enhance healing that can help process bone regeneration. Improvement for vascular endothelial growth factor expression in the culture medium of bone marrow mesenchymal stem cells. The Purpose of this study is to determine the effect of alpha tocopherol supplementation of the expression of VEGF on hBMMSC. In this study we divided into 2 main groups: Alpha Tocopherol group and control group. Each group consist of 5 samples and was observed on the 1st, 3rd, and 7th days. Human bone marrow mesenchymal stem cell multiplicated towed to 5x104 in 96 well. The cells the supplemented with VEGF and then observed under microscope fluorescent. The results of statistical analysis based on the findings of this study were conducted using the Mann-Whitney test There was no significant difference between alpha tocopherol and control group on observation day 1 and day 7 (p=0,549 and p=0,419) but there was significant differences in the 3rd day (p=0.042). There was no increase in VEGF expression in hBM-MSC after supplemented with Alpha tocopherol on observation day 1 and 7, but there was an increased in day 3.

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