Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 205, Issue 4, 2018; Page No.(1232-1237)

ANTIMICROBIAL ACTIVITY OF TOTAL INTRACELLULAR PROTEIN ISOLATED FROM PROBIOTIC INTESTINE MICROBIOTA

PINKY LALWANI, KUMUD BALA AND YASH SHARMA

Abstract

The purpose of the present study was to investigate antimicrobial property of intracellular proteins isolated from the intestines of chicken, goat and fish. Intestine of chicken, goat and fish was collected and probiotic microbiota pure culture isolates were obtained by serial dilution and characterize by biochemical test and 16S rRNA gene sequencing (Retrieved sequences were submitted to NCBI Gene Bank). Total intracellular proteins were isolated using specific protein extraction buffer and precipitation method. SDS PAGE analysis was done in order to obtain the presence of different size of proteins. Antimicrobial activity was observed against Escherichia coli by Agar well diffusion method and their zone of inhibition was calculated. Probiotic isolate, i.e. PLKBYS_2017F, PLKBYS_2017C and PLKBYS_2017 from intestine of fish, chicken, and goat, respectively found to identified by 16S rRNA genesequencing that has revealed the Bacillus amyloliquefaciens (MG525550), Bacillus amyloliquefaciens (MG525549) and Bacillus thuringiensis (MG525548). Isolated total intracellular protein was found to be effective against Escherichia coli as observed from agar well diffusion method. From the present study it in inferred that intracellular proteins isolated from intestine of chicken, goat and fish can be utilized as a potent antimicrobial agent.

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