ISOLATION AND IDENTIFICATION OF BIOACTIVE PROTEIN FROM FISH BY PRODUCTSN. Indira, K. Valivittan and P. DhasarathanAbstract The utilization of fish byproducts is an important production opportunity for the fishing and seafood processing industry, as it can potentially generate additional income as well as reduce disposal costs for these materials. A promising anticancer peptide was also obtained from shrimp shells and was shown to significantly inhibit the growth of both colon and liver cancer cells. The fish proteins are isolate from the fish muscles, gas chromatography was done to characterize the proteins extracted from the fish muscles, GC/MS peaks were sorted based on elution times, and averages of peak areas were determined from three replicates. GC-MS analysis showed the presence of 16 bioactive protein compounds in the extract of Cyprinus carpio. Finally results were observed through western blot in which molecular weight of the protein was determined. A distinct protein band with MW of 120 kDA was observed at rockfish muscle protein treated with acid. The identified bioactive compounds have a great potential of antibacterial activity against S. aureus and K. pneumonia and antifungal activity against A.fumigatus.
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