Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 20, Dec. Suppl. No.2, 2018; Page No.(13-16)

MOLECULAR DETECTION AND PHYLOGENETIC TREE ANALYSIS OF SALMONELLA PULLORUM ISOLATED FROM BROILER CHICKEN IN AL-DIWANYIA PROVINCE, IRAQ

HIBA TURKEY ATYIA AND ABBAS HADIJASIM AL-MAHMOUDI

Abstract

The current molecular-based analytical study was initiated to detect the infection occurrence of Salmonella pullorum in broiler chicken plus to understand some of the evolutional features of the study isolates and compare them with global isolates. Here, 25 cloaca fecal samples were collected from 25 broiler chickens of a breeding hall in Al-Diwaniyah City, Iraq. The samples were subjected, first, to polymerase chain reaction (PCR)-based exploring for the presence of this bacterium. Then, some of the positive samples from the PCR were sent out for partial sequencing. Both PCR and sequencing employed 16S rRNA gene as a target. The sequencing results were studied for evolution using phylogenetic analyses that were ended up with building a phylogenetic tree to compare our isolates with some global isolates of this bacterium. The PCR method showed the occurrence of S.Pullorum in 8 (32%) out 25 samples. The sequencing, for four-PCRpositive samples, confirmed the presence of this bacterium in the positive samples. When studied the phylogeny, the confirmed isolates (No.1 (MH752058), No.3 (MH752060), and No.4 (MH752061)) showed 99% similarity to the NCBI isolate, MH421997.1, an isolate from Colombia. Moreover, the current study isolate No.2, MH752059, revealed 99% matching to the NCBI isolate, MH446374.1, another isolate from Colombia. The current molecular and phylogenetic study reveals the actual present of S.Pullorum in the broiler chickens of the studied city plus shows evolutional characteristics and links to some global isolates.

Enter your contact information below to receive full paper.
Your Name :
Email:
Phone:
City:
Cost of Full Paper: Rs.75 for Indian Nationals or $20 (USD) for international subscribers.
By clicking on Request Paper you Agree to pay the above mentioned cost per paper.
  
Journal Issues
Vol 20, Dec. Suppl. No.2, 2018
Vol 20, Issue 4, 2018
Vol 20, Dec. Suppl. Issue, 2018
Vol 20, Oct Suppl. Issue, 2018
Vol 20, Issue 3, 2018
Vol 20, Issue 2, 2018
Vol 20, Issue 1, 2018
Vol 20, Feb. Suppl. Issue, 2018
Vol 19, Issue 4, 2017
Vol 19, Nov. Suppl, Issue 2017
Vol 19, Issue 3, 2017
Vol 19, Issue 2, 2017
Vol 19, Issue 1, 2017
Vol 18, Issue 4, 2016
Vol 18, Issue 3, 2016
Vol 18, Issue 2, 2016
Vol 18, Issue 1, 2016
Vol 17, Issue 4, 2015
Vol 17, Issue 3, 2015
Vol. 17 Special Issue 2015
Vol 17, Issue 2, 2015
Vol 17, Issue 1, 2015
Vol 16, Issue 4, 2014
Vol 16, Issue 3, 2014
Vol 16, Issue 2, 2014
Vol 16, Issue 1, 2014
Vol 15, Issue 4, 2013
Vol 15, Issue 3, 2013
Vol 15, Issue 2, 2013
Vol 15 Issue 1, 2013
Vol 14, Issue 4, 2012
Vol 14, Issue 3, 2012
Vol 14, Issue 2, 2012
Vol.14, Issue 1, 2012
Vol.13, Issue 4, 2011
Vol.13, Issue 3, 2011
Vol.13, Issue 2, 2011
Vol.13, Issue 1, 2011
Vol.12, Issue 4, 2010
Vol.12, Issue 3, 2010
Vol.12, Issue 2, 2010
Vol.12, Issue 1, 2010
Vol 11, Issue 4, 2009
Vol 11, Issue 3, 2009
Vol 11, Issue 2, 2009
Vol 11, Issue 1, 2009
Vol 10, Issue 4, 2008
Vol 10, Issue 3, 2008
Vol 10, Issue 3, 2008
Vol 10, Issue 2, 2008
Vol 10, Issue 1, 2008
Vol 9, Issue 4, 2007
Vol 9, Issue 3, 2007
Vol 9, Issue 2, 2007
Vol 9, Issue 1, 2007
Vol 8, Issue 4, 2006
Vol 8, Issue 3, 2006
Vol 08, Issue 2, 2006
Vol 08, Issue 1, 2006
Vol 7 Issue 4, 2005
Vol 07, Issue 3, 2005
Vol 07, Issue 2, 2005
Vol 6 Issue 4, 2004
Vol 6 Issue 3, 2004
Vol 6 Issue 2, 2004
Vol 6 Issue 1, 2004
Vol 5 Issue 3, 2003
Vol 5 Issue 2, 2003
Vol 04, Issue 4, 2002
Vol 04, Issue 1, 2002
Vol 3 Issue 1-2, 2001
Vol 1 Issue 1-2, 2000
Vol 1 Issue 3-4, 1999
Vol 1 Issue 1-2, 1999
Looking for Past Issues? Click here to get them!!