Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

MOLECULAR DIAGNOSIS OF BRUCELLOSIS IN CAMELS BY CONVENTIONAL POLYMERASE CHAIN REACTION IN WASIT PROVINCE, IRAQ

BASIM MOHAMMED HANON AND ABBAS HASSAN KHLATY AL-SARY

Abstract

Adopting a cross-sectional study design the current research aimed to determine the occurrence of the seroepidemiological infection brucellosis in camels in wasit province of Iraq from November 2016 to April 2017. Brucellosis is one of the most common diseases among animals and human. Camel brucellosis represents a major public health concern, which affects social and economic development in developing countries. Blood samples were collected randomly from animals in the herds. 237 samples from animals test by Rose Bengal Plate Test (RBPT) and indirect enzyme linked immunosorbent assay (ELISA) kits. Total two hundred thirty seven 237 samples were collected from 6 different herd the sample were taken from camels 209 females and 28 males, all samples were examined by (RBPT), the prevalence of brucellosis depend of agglutination tests to determine the positive results out of 237 collected 51 sample were positive for RBPT and 186 sample negative results, while among 51 samples positive by RBPT confirmed by ELISA 39 sample were positive and 198 sample were negative, This positive sample divided in to 6 livestock groups, from 1 to 6 groups (5) 17.25%, (4) 11.10%, (8) 20%, (7) 17.5%, (10) 13.4%, (5) 18.5%, respectively with final percentage 16.29% while the negative result percentage 83.71%, high seroprevalence was recorded in moderate age (24) 10.12 % and the older than 10 years age (9) 3.79% while the less percentage in group 1under 5 years of age (9) 2.53 %, while the seroprevalence recorded higher percentage in females (1) 97.43 % and less recorded in male (1) 2.57%. While the molecular detection of 2.95% for Brucella abortus and 2.1% for Brucella melitensis respectively. Camels are highly susceptible to brucellosis caused by Brucella melitensis and Brucella abortus. Clinical signs alone are not sufficient for brucellosis diagnosis. Difficulties can arise in diagnosis of camel brucellosis, especially as this disease provokes only few clinical signs in clinical course.