Pollution Research Paper

Vol 38, March Suppl Issue; Page No.(109-114)

THE EFFECT OF POLLUTANT CONCENTRATION ON OIL SLUDGE BIODEGRADATION USING BACTERIAL CONSORTIUM IN BIOSLURRY REACTOR

NI'MATUZAHROH, LAILATUL MIKROZIAH, NUR INDRADEWI OKTAVITRI, NASTITI TRIKURNIADEWI, FATIMAH1 AND HANIF YULIANI

Abstract

Bio-slurry reactor is one method of oil sludge treatment technology. Its application in the field requires a study of the exact comparison between pollutant content and hydrocarbonoclastic microbes, and this is still very poorly done. This study aims to determine the effect of pollutant concentration on Total Petroleum Hydrocarbon (TPH) and Total Plate Count (TPC) during biodegradation of oil sludge. Biodegradation by bio-slurry method was done by dissolving the pollutant into the liquid phase with a ratio of 1:9. Variations of pollutant concentrations were 20%, 30%, and 40% (v/v). Bacterial consortium consist of Bacillus sp., Micrococcus sp., Pseudomonas sp., and Acinetobacter sp. in the same ratio was used of 5% (v/v). Biodegradation was conducted at ± 30 oC for 14 days incubation with aeration. Variation of pollutant concentration gave effect on reduced of TPH and total amount of bacteria. Treatment with 20% pollutant concentration and addition of bacterial consortium was able to reduce hydrocarbon oil sludge up to 0.3 g/g. Percentage of biodegradation was obtained 58.9% and total bacteria was obtained log 17.5 CFU/mL after 14 days incubation. The pH conditions during biodegradation ranged from 5.0 to 7.2. Proportion between pollutants and microbial concentrations determines the success of biodegradation.

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