Ecology, Environment and Conservation Paper

MOLECULAR STUDY OF VIRULENCE FACTORS INFLUENCING THE PATHOGENICITY OF TRICHOPHYTON RUBRUM

Majid K. Alshibly and Ali M. Al-Marshedy

Abstract

The study includes collect (70) samples of skin and their components were collected randomly and (50) positive samples of nematode fungus were isolated. T. rubrum (25) gave a positive sample which formed half of the nematode fungus and was distributed between skin, hair and nails (7, 8, and 10) isolate respectively. The results of the study included testing of the hair hole that gave a negative result, and the test of urea degradation. The results were negative or heterogeneous between the isolates. The growth test on the center of the PDA was positive, while the production of the Chlamydiaspores was rare in this fungus. The virulence factors that enable the fungus to penetrate host tissues were studied during leucine aminopeptidase (LAP) and subtilin family (1-7) (SUB). It was observed that there were no significant differences in gene isolates of T. rubrum at significant level 5 Where the SUB4 gene recorded the highest percentage by 90% while the SUB6 gene recorded the lowest percentage by 30%.