Ecology, Environment and Conservation Paper

RESULTS OF CATTLE EXAMINATION FOR CAMPYLOBACTERIOSIS USING POLYMERASE CHAIN REACTION METHOD

Sergey Nikolaevich Borovikov and Madina Sailaubekovna Aitmagambetova

Abstract

Cattle campylobacteriosis is widely spread, including the Republic of Kazakhstan, and causes economic losses associated with abortions, the imposition of restrictive measures, and expenses for veterinary medical preparations and disinfection. This work shows the results of examining the animals from separate farms in the Akmola region using the polymerase chain reaction (PCR). For the research, biological material (vaginal mucus, milk, and feces) was taken from cows; this material was used for detecting pathogen Campylobacter fetus subsp. fetus by real-time PCR. DNA was extracted using classical methods. The primers were a set of species-specific primers GC1 and GC2. The research allowed identifying the agent of the disease in three samples of vaginal mucus taken from the same farm.