Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 21, Issue 1, 2019; Page No.(114-123)

ISOLATION AND SCREENING OF LIGNIN DEGRADING FUNGUS FOR BIODEGRADATION OF AGE OLD RICE STRAW

ANURADHA MUKHERJEE, ANAMICA BHATTACHARYA, TAMAL MANDAL AND PRADIP K. CHATTERJEE

Abstract

This study aims to isolate and screen lignin degrading fungus from nearby locality (leaf litters, decaying wood, and cow dung) which can degrade lignin during the biological treatment of the substrate to obtain monomeric sugars for bioconversion to ethanol. Samples collected from dead and decaying wood surfaces, decaying humus in the soil, decaying leaf litter and cow dung, screened by qualitative assay to determine the presence of lignin degrading enzymes. Among all the isolates grown, 4 species showed a positive indication for presence of lignin degrading enzyme by distinctly producing brownish halo around and under the colony on guaiacol supplemented plates resulted from guaiacol oxidation. Lignin degrading enzyme activity was checked by quantitative assay which confirmed the presence of lignin peroxidase, manganese peroxidase and laccase. Furthermore each isolates were separately screened by shake flask experiment using old rice straw as lignocellulosic feedstock for 4 weeks and the sugar content released in the hydrolysate was measured each week. Total reducing sugar yield was found to be maximum at 21st day as 107 mg/g when treated with the isolated strain S7. Lignin % from the dried biomass was estimated with evidence of biodegradation of lignin from 9.8 % to 6.3 %.

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