Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 21, Issue 1, 2019; Page No.(152-156)

A STUDY ON ANTIMICROBIAL ACTIVITY OF METHANOLIC PLANT EXTRACT AND SYNTHESIZED SILVER NANOPARTICLES USING TRIDAX PROCUMBENS

M. IFRAN, R. SHANMUGAPRIYA, D.R. DARJILY AND S. PRIYA

Abstract

The aim of this work is to evaluate the antimicrobial activity of Tridax procumbens, plant extract and synthesized silver nanoparticles. The synthesized Silver nanoparticles showed a change in colour from yellow to brown and had an absorption peak at 240 nm. Antibacterial activity: Four medically important bacteria was used as test isolates and synthesized silver nanoparticles exhibited low zone of inhibition when compared to Methanolic plant extract. Four potential fungal pathogens were used as test isolates. Methanolic plant extract exhibited higher antifungal activity than the synthesized silver nanoparticles. MIC of plant extract for Pseudomonas sp. E.coli and Staphylococcus aureus was found to be 12.5 mg/mL; MIC of Tridax procumbens for Fusarium sp. Mucor sp. was found to be 12.5 mg/mL whereas for Aspergillus sp. and Pencillium sp. the MIC was found to be 25 mg/mL. Our results revealed that Methanolic extract of Tridax procumbens was effective against the clinical pathogens. The Methanolic extract showed significant antibacterial and antifungal activity than the synthesized silver nanoparticles. Further the mechanism of action and the active compounds of the plant extract need to be elucidated for its active usage as a therapeutic drug. This study helps to exploit various plant extract and test its antimicrobial property. Further this work will include exploring the chemotherapeutic property of various available medically important plants for its usage as a chemotherapeutic drug. This work will form a platform for the usage of extract against the multidrug resistant pathogens so that the development of multidrug resistance could be prevented. This plant extract will also replace the use of synthetic chemotherapeutic drug.

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