Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 21, Issue 2, 2019; Page No.(363-368)

THE GENOTYPE OF AQUILARIA MICROCARPA INTERACTING WITH FUSARIUM SP BASED ON MICROSATELLITE MARKER

RIMA H.S. SIBURIAN, U. SIREGAR, I. SIREGAR AND ERDY SANTOSO

Abstract

Aquilaria microcarpa is one of the potential trees species for producing agarwood. The formation of aloes only occurs when the plant interacts with pathogenic substance. The process of a secondary metabolite is strongly influenced by gene activities and environmental factors as supportive component. However, the genotype information of A. microcarpa is still lacking. The genotype of A. microcarpa interacting with Fusarium sp is expected to be produced by using microsatellite markers in order to obtain the molecular characters of the plant. This result indicates that the process of morphological change occurs one week after plants were inoculated by Fusarium sp which were characterized by discoloration of leaves. With amplification testing of DNA fragments by four pairs of microsatellite primers in the population of A. microcarpa population, it was found that only primers: 10 PA 17 and 16 PA 17 were capable to amplify 5 to 6 polymorphic alleles. The molecular information is pivotal and would be used to consider the development of its culture and conservation strategic in nature.

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