Ecology, Environment and Conservation Paper

EXPLORATION STUDY OF THE PIGMENT TYPES OF STONE CRAB(GRAPSUS ALBOLINEATUS) AS PHARMACEUTICAL AND COSMETICS INGREDIENTS

Darus Saadah J. Paransa, Desy M.H. Mantiri, James J.H. Paulus, Cysca Lumenta, Medy ompie,Suria Darwisito, Carolus Paruntu, Alfread Kase and Anggreni D.N. Rupidara

Abstract

Grapsus albolineatus is a type of crustacean on the rocky coast of tropical regions like Indonesia. Some of the distribution areas of this crab species are the rocky coastline of, Tanawangko and Manggatasik Village of,Manado City, North Sulawesi. Grapsus albolineatus is known to have a high content of carotenoid pigment.Carotenoid pigments are useful for human needs, such as immune substances, and various industrial raw materials such as pharmaceutical, cosmetic and foodstuff industries. In this study, identification of the pigment type was performed on the carapace, gonad, blood, epidermal layer, and hepato pancreas using Thin Layer Chromatography method. The identification of pigments on the carapace was using Column Chromatography and High-Performance Liquid Chromatography (HPLC) methods. The results showed that Grapsus albolineatus was identified at the D molting stage. In the Thin Layer Chromatography analysis,there were found some pigment types* namely -Carotene, Echinenone, Canthaxanthin, Cryptoxanthin,Adonirubin, Astaxanthin and Astacen. Meanwhile, the separation by Column Chromatography methodshowed the results of pigments such as -Carotene, Canthaxanthin, Adonirubin, and Fucoxanthin. Theconcentrations of pigments contained in the carapace, epidermal layer, hepatopancreas, gonad and blood were respectively 6.88 mg/g, 210.47 mg/g, 454.0 mg/g, 433.33 mg/g, and 153 mg/g. The pigment content of each organ of G. albolineatus crabs was 6.95 mg (the carapace), 7.26 mg (epidermal layer), 7.07 mg(hepatopancreas), 34.94 mg (gonad), and 5.86 mg (hemocyanin).