Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper


Vol 14, Issue 3, 2012

Page Number: 325-331

EXTRACELLULAR GLUCOAMYLASE FROM THE ISOLATE STREPTOMYCES ALBOLONGUS (A5)

T. TAZNIN AND M.N. AN WAR

Abstract

Three Streptomyces isolates (A2, A5 and RB20) were collected from the Department of Microbiology, University of Chittagong, Bangladesh. Among them isolate A5 exhibited highest amylase production at 37°C temperature, medium pH 7.0 and 96 hrs of incubation period. 1.5% carbon (starch) and 1% nitrogen (yeast extract) was found to induce amylase production by the isolate Streptomyces albolongus (A5). Maximum glucoamylase activity was observed at 50°C temperature for 90 minutes and pH 7.0 along with 1% soluble starch as substrate concentration during enzyme substrate reaction.