P. MUTHUKUMAR, T.S. BLESSY THAYALIN AND V. PRABAKARAN
Abstract
Lactic acid bacteria were isolated from the raw milk sample. About six different Lactobacillus species were identified phenotypically and they were named as LAB RD1, LAB RD2,LAB RD3, LAB RD4, LAB RD5, and LAB RD6. These isolates were grouped on the basis of gram staining, cell shape, production of gas from glucose and catalase activity. Based on the phenotypic characteristics, all the isolates were gram positive rods, catalase negative and lactose fermenters (Table 1). The isolated Lactobacilli were tested for resistance to bile salt, acidic pH, NaCl, as well as their ability to inhibit pathogens. A clear zone was found around the isolate which confirms the production of acid, by dissolving the CaCO3 in the medium.