BHUSHANAM M, MADHUSUDHAN S. AND ARUN JYOTHI MATHIAS
Abstract
Honey is a natural gift to man from Mother Nature which is made available to us from themysterious kingdom of the bees. The aim of the present study was to evaluate the synergistic antibacterialeffect between the Apis honey and common antibiotic ciprofloxacin on multiple antibiotic resistant bacteria.Fifteen honey samples of Apisflorea were harvested from various geographical areas of Coorg, Karnatakaduring 2020-2021. The honey samples and ciprofloxacin were subjected to Determination of antibacterialpotency by Antibacterial disc diffusion assay separately against the test bacteria. Then followed Screeningfor synergistic activity and Data Analysis. The findings of the present study revealed that synergisticantibacterial sensitivity with inhibitory zones that were formed in the cultures against the selected bacterialstrains showed significant variations. The highest antibacterial activity was recorded against Staphylococcusaureus (ATCC 6538) with 19.26 ñ 0.23 mm. However, the least sensitivity range was recorded for Erwinianigrifluens (ATCC 21922) with 8.97ñ 0.48 mm. Bacillus cereus (ATCC 31443) showed mm, ZI of 13.74ñ0.37,Bacillus subtilis (ATCC 32441) with 12.99ñ0.27,Burkholderiaglumae (ATCC 25813) with 11.87ñ 0.58, E.coli(ATCC 25891) with 14.95ñ 0.41 mm, Klebseillasp.(ATCC 31482) with 12.11ñ 0.59, Pseudomonas aeruginosa(ATCC 287858) with 17.53ñ 0.72 mm. The research findings of the present study on the synergisticantibacterial activity of ciprofloxacin and Apisflorea honey on pathogenic bacteria showed potentialusefulness of honey and antibiotics together in the clinical practice against the pathogenic effects of strainsof bacteria.