Anupa, T., Somkuwar, R.G and A.K. Sharma and Alok Gupta
Abstract
The current study, titled âStudies on in vitro Surface sterilization and disinfection procedure for in vitro propagation of grape rootstocks was conducted at the Tissue Culture Laboratory, ICAR-National Research Centre for Grapes Pune, from 2018-19 and 2019-2020. maximum explant establishment (82.3 %) and (80.3 %) were recorded in Dogridge and 110 R, respectively. When compared to May, June and July, the month of âAprilâ is ideal for culturing nodal cuttings. Both rootstocks had a similar pattern in terms of the monthto- month establishment. Carbendazim @ 1 g/l for 2 hours was shown to be the most effective in reducing contamination and promoting plant establishment in Dogridge (11.6 %) and 110R (13.3) rootstocks respectively. In Dogridge and 110 R rootstocks, charcoal @ 1g/l was found to be effective in reducing browning (12.3 and 12.6 %) and contamination rate (11.3 and 11.0 %). In Dogridge and 110 R. Surface sterilization with mercuric chloride for 7-10 minutes was shown to be effective in reducing contamination rate for nodal explants in Dogridge (19.6 %) and 110 R (22.6 %).