PUKHRAJ MEENA, MOTILAL, DOOMAR SINGH, NAHID USMANI AND SHAILENDRA YADAV
Abstract
Microbial principle-based biosensor containing immobilized enzyme tyrosinase has been used for detection of polyphenols in tea solutions. The immobilized tyrosinase based biosensor could detect polyphenols in the concentration range 10-80 mmol L-1. Immobilization of the enzyme by the cross-linking method gave good stable response to polyphenols. The biosensor was tested in the polyphenols compounds determination in vegetables extract and the results were compared with the FolinâCiocalteau traditional method and the biosensor showed suitability to the quantification of the total polyphenol in the tested samples. In order to enhance the sensitivity and stability of the measurements, the electrodes modified with designable molecules have been used in electrochemical determination of polyphenolic compounds. Although the majority of modified electrodes successfully determined the concentrations of polyphenolic compounds, the fabrication of the electrochemical sensor is still one of the challenging tasks for the researchers.