S. Sofia, D. M. Reddy, K.H.P. Reddy, P. Latha, B. Ravindra Reddy and M. Sreevalli Devi
Abstract
Mutagenic effectiveness and efficiency of gamma rays, ethyl methane sulphonate (EMS) and sodium azide (SA) treatments were assessed in two mungbean varieties viz., WGG-42 and LGG-460 in M2 generation. Mutagenic effectiveness and efficiency were calculated in relation to biological damage in M1 and viable mutations in M2 generation. In the present study, viable mutation frequency increased with an increase in dose/concentration of mutagens, EMS showed the highest frequency of viable mutations than gamma rays and SA treatments in both the genotypes. The highest frequency of viable mutations were observed at 600 Gy of gamma rays, 0.6% of EMS and 3 mM of SA. The mutagenic effectiveness and efficiency showed a trend, which was inversely proportional to the increasing dose of mutagens used, EMS treatments were found to be more effective and efficient in inducing viable mutations followed by gamma rays and SA treatments in both the cultivars.