Jeevitha S. and Anandan R.
Abstract
Cassia species are considered as a valuable source for our Indian medical system. Inter and intra species of Cassia is genetically variable and its necessary to identify and discriminate individuals in order to exploit its maximum potential. DNA barcode technique of well-studied universal markers were used to amplify the genomic region of several Cassia species. The amplified regions were sequenced and the codingâs were blasted and studied for any significant differences. The results from the generated Rbcl, MatK and Ycf1b sequences were almost identical to Senna species that is closely related to Cassia species. The phylogenetic tree for five species of Cassia was constructed using three markers (Rbcl, MatK and Ycf1b). Among the markers used Rbcl_1 shows the best resolution for S. auriculata. MatK_3 has showed better species resolution for S. occidentails than other markers. The implication of these results can be used in species identification of wild and native genotypes of medicinal plant from various geographical locations.