Suraj Jadhav, Suresh Jagtap, Anand Zanwar and E. A. Singh
Abstract
The âSarivaâ, a drug having diverse uses in Ayurveda. Under the name âSarivaâ four different species have been mentioned and supplied viz. Cryptolepis buchananii Roem & Schult., Decalepis hamiltonii Wight. & Arn., Hemidesmus indicus (L.) R.Br., and Ichnocarpus frutescence R. Br. Vanillin is a key active constituent of these plants. Considering the availability of plant material in the market there is an ambiguity in supplying the authentic species as âSarivaâ. This study was planned to study the effective species as âSarivaâ using biological activity as well as content of Vanillin using HPTLC fingerprints, and antioxidant abilities. A precise method was developed for detecting vanillin concentrations in various âSarivaâ extracts, using suitable mobile phase. The method was suitable for routine examination of vanillin in âSarivaâ spp. providing reliable and consistent results. The highest peak area for vanillin was reported in Hemidesmus indicus, followed by Decalepis hamiltonii, Ichnocarpus frutescence, and Cryptolepis buchananii based on area under curve, indicating content variation in tested samples. The study also assessed the antioxidant capability of different âSarivaâ drugs using DPPH, FRAP, and ABTS assays. Hemidesmus indicus showed significantly higher antioxidant activity followed by Decalepis hamiltonii, Cryptolepis buchananii and Ichnocarpus frutescence. These findings suggest that Hemidesmus indicus and Decalepis hamiltonii have similar natural antioxidants with potential health benefits. Considering the less availability of Hemidesmus indicus, Decalepis hamiltonii can be potent substitute as âSarivaâ. Further research is needed to explore the specific mechanisms of action and potential applications of Vanillin in preventing oxidative stress-related diseases.