E. Anusha and E.M. Manogem
Abstract
For thousands of years, medicinal plants were used to cure many common infectious diseases and health issues. An extremely important medicinal plant called Michelia champaca L.(Magnoliaceae) has long been used to treat various ailments, including inflammatory ones. The current study was designated to explore the antibacterial properties of the alcoholic and methanolic extract of Magnolia champaca leaves and its different fractions. Antibacterial activity was evaluated against a panel of gram-positive (Staphylococcus aureus, Enterococcus faecalis, Staphylococcus saprophyticus) and gram-negative (Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, Proteus mirabilis, Acinetobacter baumannii, and Salmonella paratyphi) bacterial strains using the disc diffusion method. According to the results, methanolic extract showed slightly higher antibacterial activity than acetone extract. The highest zone of inhibition in the methanolic extract was Staphylococcus aureus followed by Staphylococcus saprophyticus and Proteus mirabilis. The acetone extract shows significant efficiency in gram-positive strains such as Staphylococcus spp. The FTIR spectroscopic studies revealed the presence of various functional compounds such as alkanes, carboxylic acid, aromatic compounds, esters, amines, nitro, alkyl halide, and phenols. Acetone and methanol extracts showed the presence of halo compounds, anhydride, isothiocyanate, alcohol, and phenol. GCMS analysis of both extracts shares five biocomponents: Alpha copaene, beta-elemene, beta-caryophyllene, and phytol showed antibacterial activity. This investigation showed that M. champaca leaf extracts had prospective therapeutic properties in terms of their antibacterial properties.