Bhaba Kumar Pegu, 1Limpon Bora, Jyoti Prava Engty and Devid Kardong
Abstract
The study aims at screening, isolation, and characterization of naringinase enzyme producing bacterial strains from soil samples. In this study, 45 bacterial isolates were screened for naringinase activity, and among them, 20 bacterial isolates are found to be capable of naringinase production in liquid mineral medium. Bacterial isolate D1 exhibited the highest naringinase activity in liquid mineral medium. Based on the morphological, biochemical, fatty acid profile, and molecular characterization, bacterial isolate D1 was identified as Bacillus amyloliquefaciens. Impact of different submerged culture parameters were evaluated in different experimental conditions. The study revealed that 48 hrs of incubation periods, pH 6.0 and temperature 35 0C is optimal for naringinase activity when the culture medium is supplemented with starch and yeast extract as carbon and nitrogen sources. Thus, it can be concluded that due to the potential naringinase activity of the strain Bacillus amyloliquefaciens D1, further the identifying strain may be used in citrus processing and bioprocess industries upon large scale field applications.